Sucrose and water are mixed in E-flasks in ratio liquid:solid 10:1. Temperature is kept constant at 60° C. in an oven equipped with a shake table. Succinic acid is added to the flasks to give concentrations of 1, 3, and 5% acid by weight. The data is shown in Tables 5 to 7. An increase in the catalyst loading increases the rate of hydrolysis significantly at low temperatures. Yields after ten (10) hours hydrolysis for glucose and fructose are summarized in Table 1. The yields are based on the amount of glucose or fructose formed divided by the amount of sucrose hydrolyzed. For glucose at higher acid concentrations some product degradation takes place. For fructose some degradation is evident at all acid concentrations.

The fermentation byproduct, acetate, was completely separated from succinate. A simple hot water regeneration successfully concentrated succinate from 10 g/L (inlet) to 40-110 g/L in the effluent. If successful, this would lower separation costs by reducing the need for chemicals for the initial purification step. Despite promising initial results of good capacity (0.06 g succinic/g sorbent), 70% recovery using hot water, and a recovered concentration of >100 g/L, this regeneration was not stable over ten cycles in the column. Alternative regeneration schemes using acid and base were examined and more sorbent screened. Tests were performed with both simulated broth containing succinic acid(cas:110-15-6) at various concentrations and with actual broth provided by MBI. Seven of the most promising resins were tested for regenerability and stability using a modified extraction procedure combining acid and hot water washes. Two (XUS 40285 and XFS) showed both good stable capacities for succinic acid over ten cycles and more than 95% recovery in a batch operation. These results indicate that sorption for succinic acidsuccinic acid(cas:110-15-6) continues to be promising. However, it will need additional column and process tests focusing on the regeneration of the resins and the concentration of the succinic acid in the effluent streams