In the second feedstock production strategy, flour hydrolysis conducted by mixing fungal broth filtrate with wheat flour generated a glucose-rich stream, while the fungal bio-mass was subjected to autolysis for the production of a nutrient-rich stream. The possibility of replacing a commercial semi-defined medium by these two streams was investigated sequentially. A. succinogenes fermentation using only the wheat-derived feedstock resulted in a succinic acid concentration of almost 16 g l(-1) with an overall yield of 0.19 g succinic acid per g wheat flour. These results show that a wheat-based bio-refinery employing coupled fungal fermentation and subsequent flour hydrolysis and fungal autolysis can lead to a bacterial feedstock for the efficient production of succinic acid(cas:110-15-6).

Sucrose and water are mixed in E-flasks in ratio liquid:solid 10:1. Temperature is kept constant at 60° C. in an oven equipped with a shake table. Succinic acid(cas:110-15-6) is added to the flasks to give concentrations of 1, 3, and 5% acid by weight. The data is shown in Tables 5 to 7. An increase in the catalyst loading increases the rate of hydrolysis significantly at low temperatures. Yields after ten (10) hours hydrolysis for glucose and fructose are summarized in Table 1. The yields are based on the amount of glucose or fructose formed divided by the amount of sucrose hydrolyzed. For glucose at higher acid concentrations some product degradation takes place. For fructose some degradation is evident at all acid concentrations.

To produce a media with high enough sugar concentration for fermentation purposes higher liquid:solid ratios (higher percentages of sucrose) must be used. Using the same experimental setup as in Example 2, but with liquid:solid ratio 10:4, acid concentrations of 1%, and 5% were investigated at 80° C. and 100° C. Sucrose hydrolysis results are presented in Table 14. Yields after finished hydrolysis are shown in Table 2. The yields are expressed on a weight basis as the mass of produced monosaccharides per mass of hydrolyzed sucrose. From the yields it can be seen that the best results are achieved by low acid loadings and high temperatures. After one hour the yields were close to 100%, and heating for another hour did not generate any significant sugar degradation.