The of from flour was investigated in a two-stage bio-process. In the first stage, flour was converted into a generic microbial feedstock either by fungal alone or by combining fungal for enzyme and fungal bio-mass production with subsequent flour hydrolysis and fungal . In the second stage, the generic feedstock was converted into by bacterial by. Direct of the generic feedstock produced by fungal alone resulted in a lower production, probably due to the low and concentrations in the fungal broth filtrate.

In the second feedstock production strategy, flour hydrolysis conducted by mixing fungal broth filtrate with flour generated a -rich stream, while the fungal bio-mass was subjected to for the production of a nutrient-rich stream. The possibility of replacing a commercial semi-defined medium by these two streams was investigated sequentially. using only the -derived feedstock resulted in a concentration of almost 16 g l(-1) with an overall yield of 0.19 g per g flour. These results show that a -based bio-refinery employing coupled fungal and subsequent flour hydrolysis and fungal can lead to a bacterial feedstock for the efficient production of .

The biosynthesis of succinic acid from wheat flour was investigated in a two-stage bio-process. In the first stage, wheat flour was converted into a generic microbial feedstock either by fungal fermentation alone or by combining fungal fermentation for enzyme and fungal bio-mass production with subsequent flour hydrolysis and fungal autolysis. In the second stage, the generic feedstock was converted into succinic acid(cas:110-15-6) by bacterial fermentation by Actinobacillus succinogenes. Direct fermentation of the generic feedstock produced by fungal fermentation alone resulted in a lower succinic acid(cas:110-15-6) production, probably due to the low glucose and nitrogen concentrations in the fungal broth filtrate.

In the second feedstock production strategy, flour hydrolysis conducted by mixing fungal broth filtrate with wheat flour generated a glucose-rich stream, while the fungal bio-mass was subjected to autolysis for the production of a nutrient-rich stream. The possibility of replacing a commercial semi-defined medium by these two streams was investigated sequentially. A. succinogenes fermentation using only the wheat-derived feedstock resulted in a succinic acid(cas:110-15-6) concentration of almost 16 g l(-1) with an overall yield of 0.19 g succinic acid(cas:110-15-6) per g wheat flour. These results show that a wheat-based bio-refinery employing coupled fungal fermentation and subsequent flour hydrolysis and fungal autolysis can lead to a bacterial feedstock for the efficient production of succinic acid(cas:110-15-6)