Succinic acid
Succinic acid
disodium succinate
Phthalocyanine pigment
Compound dyes
Compound green
Composite blue
Succinic Acid(cas:110-15-6) Production Utilizing Industrial Hydrolysate
Release time:2016/7/22 15:25:25

AFP 184 was placed in a fermenter with true hydrolysate, from rice straw. An exemplary hydrolysate is that commercially prepared and made available from Arkenol Inc., of Mission Viejo, Calif., via its concentrated acid hydrolysis process. The rice straw medium contains approximately 600 g/L glucose and 169 g/L xylose as the two main sugar components, plus minor quantities of other sugars. The experimental data are found in Table 2 and in FIG. 2.

The following is a protocol of the AFP 184-based fermentation process:

The fermentation medium contained the following components: Difco yeast extract 5 g/L, tryptone 10 g/L, (NH4)2SO4 2 g/L, MgSO4—7H2O 0.2 g/L, NaCl 10 g/L, K2HPO4 7 g/L, KH2PO4 3 g/L, Arkenol's hydrolysate 16.5 mL/L, and kanamycin 30 mg/L. The industrial hydrolysate contained 607 g/L glucose and 169 g/L xylose as the two main sugar components plus minor quantities of other sugars. The medium with all of the components except the antibiotic was autoclaved at 121° C. for 20 minutes. Kanamycin then was added upon cooling. This fermentation medium was used for both the inoculum flasks and the one-liter fermenter. For the inoculum, 50 mL medium was placed in a 250-mL flask and inoculated with 0.2 mL of the AFP184 stock culture which was maintained in 30% glycerol and at ?70° C. The flask was incubated in a incubator shaker at 37° C. and 250 rpm overnight (about 16 hours).

The entire flask contents then were used to inoculate the fermenter which was maintained at 37° C. The medium in the fermenter was aerated to allow fast growth of the organism. After six hours when the required cell mass was achieved, the following actions were taken: 1. Air was turned off to exert anaerobic conditions, which would initiate production of succinic acid; 2. Carbon dioxide gas was sparged into the medium at a rate of 0.03 mL per minute; and 3. A feed solution which contained the Arkenol's hydrolysate diluted with deionized water to a concentration of 500 g/L of total glucose plus xylose was added to the fermenter to achieve a total sugar concentration of 50 g/L in the fermentation medium. During the course of the experiment, when the sugar concentration in the fermnetr was low, more feed was added to provide sufficient substrates for succinic acid(cas:110-15-6) production. As the cells produced succinic acid the pH dropped. It was maintained at pH 6.5 by addition of a 1.5 M Na2CO3 solution through the action of an automatic pH controller. Samples were taken at intervals and analyzed for optical density, glucose, xylose, succinic acid(cas:110-15-6), acetic acid, lactic acid, and ethanol.

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