Bio-oil based biorefinery strategy for the production of succinic acid. Succinic acid(cas:110-15-6) is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts.

The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid(cas:110-15-6) was investigated.

RESULTS: The transgenic E. coli strain could grow in modified M9 medium containing 2002v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.3802g/L succinic acid(cas:110-15-6) was produced. With the presence of 402g/L glucose in the medium, succinic acid(cas:110-15-6) concentration increased from 1.4 to 2.402g/L by addition of 2002v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.302g/L succinic acid was produced.

The obtained succinic acid(cas:110-15-6) concentration increased to 11.502g/L when 12.502v/v% AP-bio-oil was added. However, it decreased to 802g/L when 5002v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli. CONCLUSIONS: The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid(cas:110-15-6) production.